Crystal structure of Cas9 in complex with guide RNA and target DNA.
Abstract The CRISPR-associated endonuclease Cas9 can be targeted to specific genomic loci by single guide RNAs (sgRNAs). Here, we report...
Rapid and highly efficient mammalian cell engineering via Cas9 protein transfection
Abstract CRISPR-Cas9 systems provide a platform for high efficiency genome editing that are enabling innovative applications of mammalian...
